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業績詳細

業績項目 学術雑誌論文(学内)
タイトル・書名 Improved Long-term Culture of Epidermal Stem Cells Utilizing CD200R-expressing Feeder Cells
著者
学部・講座・部門
施設
TOYA Etsuko
2015  年度
大学 講座なし 腫瘍分子生物学研究所
2015  年度
旗の台校舎
著者
学部・講座・部門
施設
OHBA Motoi
2015  年度
大学 講座なし 腫瘍分子生物学研究所
2015  年度
旗の台校舎
雑誌名 Showa Univ J Med Sci
巻・号 27 巻      2 号
開始・終了ページ 開始 83  ページ /  終了  91  ページ
刊行年月 2015 年 06 月
言語 英語
内容記述 Tissue stem cells have great potential as a source of tissue for regenerative medicine. Epidermal stem cells (EpSCs) are the most accessible population of tissue stem cells that can regenerate the specialized cell types of tissues including the epidermis, smooth muscle and the sciatic nerve. However, the difficulties in isolation of the high numbers of EpSCs and their long-term culture have hampered the development of wider clinical applications of EpSCs. Here, we present a novel approach to EpSC culture that utilizes a feeder layer of Swiss 3T3 cells expressing the putative EpSC niche-specific molecule, CD200R. The colony forming efficiency of CD34+, α6-integrin+ EpSCs was increased on CD200R-expressing Swiss 3T3 feeder cells compared with normal Swiss 3T3 feeder cells. Furthermore, treatment with glycogen synthase kinase (GSK)-3 inhibitor, an activator of Wnt signaling, synergistically enhanced the proliferation of EpSCs. These results raise the possibility that an artificial microenvironment equivalent to in vivo niches will enable the persistent culture of EpSCs, thereby increasing the utility of EpSCs for tissue engineering and regeneration.
出版者 Showa University Society
ISSN 0915-6380
資源識別子URI http://researchers-achievements.showa-u.ac.jp/dispGyoseki/?item_id=1019591&item_no=1
査読 あり
論文種別 原著